Combined application of microbial inoculant and kelp-soaking wastewater promotes wheat seedlings growth and improves structural diversity of rhizosphere microbial community

Industrial processing of kelp generates large amounts of kelp-soaking wastewater (KSW), which contains a large amount of nutrient-containing substances. The plant growth-promoting effect might be further improved by combined application of growth-promoting bacteria and the nutrient-containing KSW. Here, a greenhouse experiment was conducted to determine the effect of the mixture of KSW and Bacillus methylotrophicus M4-1 (MS) vs. KSW alone (SE) on wheat seedlings, soil properties and the microbial community structure in wheat rhizosphere soil. The available potassium, available nitrogen, organic matter content and urease activity of MS soil as well as the available potassium of the SE soil were significantly different (p < 0.05) from those of the CK with water only added, increased by 39.51%, 36.25%, 41.61%, 80.56% and 32.99%, respectively. The dry and fresh weight of wheat seedlings from MS plants increased by 166.17% and 50.62%, respectively, while plant height increased by 16.99%, compared with CK. Moreover, the abundance and diversity of fungi in the wheat rhizosphere soil were significantly increased (p < 0.05), the relative abundance of Ascomycetes and Fusarium spp. decreased, while the relative abundance of Bacillus and Mortierella increased. Collectively, the combination of KSW and the plant growth-promoting strain M4-1 can promote wheat seedlings growth and improve the microecology of rhizosphere microorganisms, thereby solving the problems of resource waste and environmental pollution, ultimately turning waste into economic gain.


Physical and chemical properties of soil in the SE and MS groups
Both SE and MS treatments affected the physical and chemical properties of the soil.In the current study, the available phosphorus, potassium and nitrogen level, organic matter content, and soil pH were evaluated as the soil fertility indices.The experiment revealed (Fig. 2) that the MS treatment significantly increased the available potassium content by 39.51%, increased the available nitrogen content by 36.25%,increased the organic matter content by 41.61%, and increased the urease content by 80.56% (p < 0.05).The SE treatment significantly increased the available potassium content by 32.99% (p < 0.05).The pH, available phosphorus, available nitrogen, organic matter, urease and phosphatase activity in the SE group were increased but not significantly different from those of the control group.

Assessment of microbial abundance and diversity
The diversity of wheat seedlings rhizosphere microbial community structure in the CK, SE, and MS rhizosphere soil samples was analyzed.The sequences were clustered using UPARSE algorithm at 97% similarity level.Finally, 3756 bacterial operational taxonomic units (OTUs) and 1411 fungal OTUs were obtained.Microbial diversity increased with the increase in sequencing depth, and all the curves leveled off, indicating that the sequencing became saturated and reflected species information for most microorganisms in the samples and that the sequencing data quality was sufficient for the ensuing analysis (Fig. S1).
Shannon index reflect the community diversity in a sample.As shown in Fig. 3c, the diversity of fungal community in MS samples was significantly higher (p < 0.05) than that in CK samples, but with no significant effect on the diversity of the bacterial community.In SE samples, the diversity of the fungal community increased and the diversity of the bacterial community decreased, but neither change was significant.Sobs index reflect the www.nature.com/scientificreports/community abundance in a sample.The bacterial community abundance in SE and MS samples were reduced compared to that in CK samples, the change was not significant (Fig. 3b).The fungal community abundance in SE and MS samples were significantly higher (0.01 < p < 0.05) than that in CK samples (Fig. 3d).These results suggest that the addition of kelp-soaked wastewater and microbial inoculants can change the microbial community structure in wheat rhizosphere soil to different extent.The principal coordinate analysis (PCoA) based on Bray-Curtis distance algorithm also confirms this conclusion (Fig. S2).

Bacterial and fungal community composition in SE and MS samples
Venn diagram was used to visualize the similarity and overlap of species in the compared samples.Figure 4 revealed that the two treatment groups and the control group contained 9953 bacterial OTUs, with 105 OTUs specific to CK samples, 79 OTUs specific to SE samples, and 111 OTUs specific to MS samples.Collectively, all the sample types contained 3156 fungal OTUs, of which 125 were specific to CK samples, 128 were specific to SE samples, and 124 were specific to MS samples.Furthermore, 2736 bacterial OTUs and 711 fungal OTUs were shared among the sample types.Next, community histogram was used to visualize the community composition and relative abundance upon different treatments at different taxonomic levels based on the results of taxonomic analysis.Analysis of the bacterial community composition (Fig. 5a) revealed that the mixture of kelp-soaked wastewater and M4-1 bacterial suspension (MS) significantly increased the relative abundance of Arthrobacter, Bacillus, Nocardioides, and Marmoriocia in wheat rhizosphere soil.The relative abundance of Enterobacterium in wheat rhizosphere soil was significantly reduced in these samples.The analysis of fungal community composition at the genus level (Fig. 5b) revealed that the mixture of kelp-soaked wastewater and M4-1 bacterial suspension (MS) significantly increased the relative abundance of Mortierella, Aspergillus, and Stephanonectria in wheat rhizosphere soil.The relative  www.nature.com/scientificreports/abundance of pathogenic fungi, such as Fusarium, Fusicolla, Cladosporium, Neocosmospora, and Gibberella, in wheat rhizosphere soils was also substantially reduced.The analysis of relative abundance differences at the phylum level revealed only small differences in the bacterial communities in wheat rhizosphere between the treatments (Fig. 6a-c), with nine phyla detected.In the CK, SE and MS groups, Actinobacteriota (25.58%, 29.60%, 28.56%), Proteobacteria (26.33%, 23,62%, 23.54%), and Acidobacteriota (14.15%, 10.71%, 13.00%) were the dominant bacterial phyla, followed by Firmicutes (6.8%, 8.59%, 7.98%)), Chlorobacteria (8.62%, 7.20%, 7.62%), Bacteroidetes (5.0%, 7.11%, 5.75%), Myxococcus (2.89%, 2.96%, 2.68%), Methylomirabilota (2.77%, 2.59%, 2.51%), Gemmatimonadota (2.41%, 1.88%, 2.20%) and Patescibacteria (0.75%, 1.61%, 2.03%).The analysis revealed that although the SE and MS treatments did not significantly affect the abundance and diversity of bacterial community, they significantly affected the abundance and diversity of wheat rhizosphere fungal communities (Fig. 6d-f).As shown in Fig. 6d,f, the relative abundance of Ascomycota and Mortierellomycota was significantly different between MS and CK samples.The relative abundance of Ascomycota decreased from 82.09% in the CK group to 50.37% in the MS group.The relative abundance of Mortierellomycota significantly increased from 11.28% in the CK group to 42.88% in the MS group (p < 0.05, Fig. 6g).
The results of the redundancy analysis of soil bacterial community composition and environmental factors are shown in Fig. 7.The main physicochemical factors affecting soil bacterial community composition were effective phosphorus and sucrase (p < 0.05), with the former having the most significant effect on the bacterial community structure (r 2 = 0.0.6876,p = 0.028, Table S1).The redundancy analysis of rhizosphere soil fungal community composition and environmental factors revealed that the following physicochemical factors affected the soil fungal community structure: effective phosphorus, organic matter, effective potassium, pH, urease, phosphatase, and sucrase (p < 0.05); of these factors, sucrase had the most significant effect on the fungal community structure (r 2 = 0.8494, p = 0.005, Table S1).

Discussion
Kelp is a cheap and abundant resource in coastal agricultural areas.A large amount of KSW is generated during the kelp processing.The direct discharge of untreated wastewater will lead to waste of resources, affect aquatic resources and aquaculture, cause harm to the Marine environment, worsen the quality of seawater, and even endanger human health.Therefore, how to make reasonable use of KSW has both economic and environmental benefits.Here, we applied KSW at appropriate concentrations to wheat seedlings and verified the growth-promoting effects of KSW and the mixture of B. methylotrophicus M4-1 and KSW on the growth of wheat seedlings as well as the structure and diversity of the microbial community in wheat seedlings rhizosphere.We confirmed that the mixture of KSW and B. methylotrophicus M4-1 improved the soil physicochemical properties, promoted wheat growth, and significantly increased the dry and fresh weights and height of the wheat seedlings.Moreover, the combination improved the rhizosphere microbial community structure, which further promoted the growth of wheat seedlings.These findings suggest that the combination could be used as a natural fertilizer for wheat, providing an environmentally friendly approach to treat kelp wastewater.
The application of rhizobacteria, specifically PGPR, as an alternative to chemical pesticides has recently gained significant attention in the scientific community.Upon application of microbial fertilizers to the seed, plant, rhizosphere, and the soil, PGPR usually colonize the rhizosphere or plant interior and increase the supply of nutrients to the host plant, thus promoting plant growth 39 .PGPR affect plant growth by improving the physicochemical properties of the soil as well as by producing a variety of metabolites, such as phytohormones (growth hormone, cytokinin, gibberellin, and ethylene), antibiotics, siderophores, extracellular polysaccharides, and ACC deaminase 40,41 .These compounds stimulate the increase in plant root length, root surface area, and root tip number, thereby increasing nutrient uptake by the plant [42][43][44] .Of note, KSW in this study are rich in phytohormones, such as indoleacetic acid and abscisic acid.
In addition to PGPR, biostimulants can also promote plant growth and suppress plant soil-borne diseases 12,45 .The term biostimulant was first introduced in 2007 by Kauffman et al., who considered biostimulants as substances other than fertilizers that promote plant growth when applied in small amounts 46 .The KSW used in the current study contains a variety of biostimulants.Specifically, it is rich in plant growth regulators (phytohormones), minerals, and trace elements, quaternary ammonium molecules (e.g., betaines), polyuronides (e.g., alginates or fucoidans), and lipid-based molecules (e.g., sterols).Biostimulants enhance root formation and elongation, increase nutrient uptake, improve seed germination and crop establishment, increase cation exchange, reduce leaching, detoxify heavy metals, and stimulate the plant immune system in response to stressors 47 .In addition to promoting plant growth, biostimulants can suppress soil-borne diseases in plants 12,45 .
Hernandez et al. confirmed that, the number of petioles and root length of strawberry significantly increased after treatment with B. methylotrophicus 48 .A similar result was reported by Yao et al. 11 , who showed that betaine and phytohormones shortened the ripening time and increased the yield of tomato by increasing the leaf area, increasing the intensity of photosynthesis, and promoting gas exchange in the leaf.In the current study, the mixture of KSW and strain M4-1 significantly increased the dry weight and fresh weight of wheat seedlings (p < 0.05, Fig. 1), presumably because the phytohormones and other substances produced by strain M4-1 promoted the number of root tips, root surface area, and root length of wheat seedlings, thus improving the uptake and utilization of various soil nutrients and, hence, the growth of wheat seedlings.
The physical and chemical properties of the soil are critical to plant growth.The pH, water, nitrogen, phosphorus, and potassium content; and activity of various enzymes directly affect plant growth.In the current study, the addition of KSW and the combination of KSW with strain M4-1 resulted in significant changes in all soil indicators relative to CK conditions.PGPR play an important role in soil nutrient transformation, promoting the transformation of complex organic molecules, facilitating the acquisition of environmental resources by the plant, and enhancing the acquisition and assimilation of nutrients by plant 49 .In the current study, the available potassium content in MS samples was significantly higher than that in CK samples (p < 0.05, Fig. 2c).This result can be explained by the fact that organic acids produced by PGPR interact with phosphorus and potassium compounds present in the soil, which are otherwise not easily accessible by the plant, and convert these elements into available phosphorus and available potassium, respectively, thereby improving the uptake and utilization of phosphorus and potassium by the plant 50,51 .A small increased in soil pH greatly alters the solubility of silicate and changes the amount of available potassium in the soil 52 .Consistent with these data, available potassium levels in MS samples were significantly higher than those in CK samples (p < 0.05).Additionally, strain M4-1 produces organic acids that release K from mineral complexes by forming chelates with Fe 2+ , Al 3+ , Si 4+ , and Ca 2+ ions bound to K minerals 53 .
In addition to increasing the content of nutrients in the soil, PGPR and KSW improved the activity of various soil enzymes.We showed that the addition of KSW alone or together with strain M4-1 altered the activities of various soil enzymes (sucrase, urease, phosphatase, and catalase), with a significant increase in urease activity in MS samples compared to the CK group.Urease, a key enzyme that regulates soil nitrogen transformation, is mainly produced by plants and microbes and plays a key role in nutrient cycling 54 .Soil enzymes are among the most active organic components of soil aggregates 55 .They are used for the assessment of soil fertility and the rate of chemical composition change 17 .Furthermore, soil enzyme activity is often used as an indicator of microbial growth in the soil.The observed changes in the various enzyme activities in MS samples may be associated with changes in soil physical properties caused by the application of KSW and PGPR, which increased soil fertility and created an environment conducive to microbial growth, thus affecting soil enzyme activities 56 .
High-throughput sequencing is widely used in biological applications, such as the assessment of soil microbial diversity, which is a key factor affecting soil health and quality.In the current study, total DNA was isolated from wheat seedlings rhizosphere soil from different treatment groups, and the 16S rRNA and ITS regions were www.nature.com/scientificreports/sequenced.The analysis revealed that the mixture of KSW and PGPR significantly affected (p < 0.05) fungal abundance and diversity, while the mixture of KSW significantly affected (p < 0.05) fungal abundance.The KSW alone or the mixture of KSW and PGPR affected the abundance and diversity of rhizosphere bacteria, however, the effect was not significant.
At the bacterial phylum level, Actinobacteria, Proteobacteria, and Acidobacteria were dominant, followed by Firmicutes, Chloroflexi, Bacteroidetes, Myxococcota, and Mehylomirabilota, Gemmatimonadota, and Patescibacteria.Actinomycetes are the main taxa of soil microorganisms that participate in the recycling of organic matter in the environment by secreting hydrolytic enzymes and also produce metabolites that promote plant growth, such as iron carriers and growth factors 57,58 .The relative abundance of the Actinomycetes in the rhizosphere soil of wheat in the treatment group of this experiment was higher than that of the control group, indicating that KSW and M4-1 can effectively increase the nutrient level of the soil and promote plant growth.In rhizosphere soils, the Proteobacteria is often associated with the nutrient content of the soil and is involved in nitrogen fixation, organic matter decomposition and promotion of plant growth 59 .Acidobacteria tend to be associated with the nutrient content of the soil 27,60 .Acidobacteria plays an important role in soil carbon and nitrogen cycling 61 and greatly affects plant growth traits and yield.For instance, Tao et al. 62 reported that Acidobacteria and Verrucomicrobia are significantly positively correlated with grain yield of maize fertilized with different green manures (p < 0.01).Bacteroidetes produces large amounts of extracellular polysaccharides, which protect the crop root system.In addition, extracellular polysaccharides improve the rhizosphere microenvironment and promote the uptake of trace elements by the plant 63,64 .The relative abundance of Bacteroidetes in wheat rhizosphere soil in the MS group was higher than that in the CK group (Fig. 6a-c), indicating that strain M4-1 increased the nutrient content of the rhizosphere soil.
At the fungal phylum level, five phyla were detected, namely, Ascomycota, Mortierellomycota, Basidiomycota, Chytridiomycota, and Unclassified Fungi (Fig. 6d-f).Compared with CK samples, the relative abundance of Ascomycota and Mortierellomycota was significantly different in MS samples (p < 0.05, Fig. 6).Ascomycota is a phylum with the largest fungal variety, with most diverse reproductive forms and morphology, encompassing over 64,000 species 65,66 .However, some Ascomycetes can infect and damage plants 67 , including crops, leading to serious economic and health problems globally 68,69 .In the current study, the relative abundance of Ascomycota in the rhizosphere soil of wheat in MS and SE samples was notably lower than that in CK samples (Fig. 6d-f; 50.37% in MS samples, 67.95% in SE samples, and 82.09% in CK samples).The relative abundance of Ascomycota in MS samples was only 31.72% of that in CK samples.This result indicates that the addition of KSW together with strain M4-1 significantly reduced the relative abundance of the plant pathogenic fungi Ascomycota in the soil (p < 0.05, Fig. 6g).In addition to Ascomycota, the relative abundance of Mortierellomycota also differed significantly among the groups (p < 0.05, Fig. 6g).The relative abundance of Mortierellomycota was 42.88% in MS samples, 24.00% in SE samples, and 11.28% in CK samples.Mortierella is a common environmental filamentous fungus species 70 found in different soils, the rhizosphere, rivers, and lakes 71,72 .Mortierellomycota decomposes a variety of complex organic compounds and inhibits the growth and reproduction of some fungal and bacterial pathogens 73 .Furthermore, Mortierellomycota synthesizes gibberellin, indoleacetic acid, and ACC deaminase, which promote the growth of tomato and wheat 72,74 .
Figures 5 and 6 show that the fungal community structure of the MS samples differed from that of the SE samples due to the addition of B. methylotrophic M4-1 suspension to the MS treatment.Studies have shown that, B. methylotrophicus secrete several antifungal metabolites to inhibit the growth of pathogens 75 , such as inhibition of suppression of maize stalk rot 76 , tomato bacterial wilt 77 and other pathogens.B. methylotrophicus M4-1 in this study was able to secrete siderophores (37.21 mg l −1 ), protease (670.82U mL −1 ) and cellulase (36.27U mL −1 ) 78 .Cellulases and proteases can hydrolyze fungal cell walls and inhibit the growth of fungal pathogens 79 .Siderophores play a significant role in the biological control mechanism against certain phytopathogens.Siderophores bind with the iron tightly and create Fe competition in the rhizospheric zone, which reduce the bioavailable iron for the plant pathogens and decreases pathogenic microbe abundance 80,81 .In summary, the M4-1 suspension added to the MS treatment was able to disturb the microbial community structure composition of the interrhizosphere soil, especially that of fungal microorganisms.

Conclusion
The present study aimed to verify the promotion effect of KSW and strain M4-1 mixture on the growth of wheat seedlings and the effect on the rhizosphere microbial community structure of wheat seedlings.The mixture of KSW and strain M4-1 improves the physicochemical properties of the soil by increasing the content of available phosphorus, available nitrogen, available potassium, and organic matter; altering the soil pH; and enhancing soil enzyme activity compared to the control.The mixture of KSW and strain M4-1 promotes the growth of wheat seedlings and significantly increases the dry and fresh weight of wheat seedlings while improving microbial abundance and diversity in wheat seedlings rhizosphere soil, reducing the relative abundance of pathogenic fungi, and increasing the relative abundance of beneficial bacteria, further promoting the growth of wheat seedlings and improving nutrient utilization efficiency of wheat seedlings.Here, we suggest using a mixture of KSW and strain M4-1 as biostimulants for wheat seedlings, which would solve the problem of difficult treatment of KSW in production, conserve agricultural water, and also alleviate the environmental pollution caused by direct discharge of KSW.

Ethics
The collection of plants material complies with relevant institutional, national and international guidelines and legislation.The samples in the study were collected on private land where the owner allowed the study.The www.nature.com/scientificreports/experimental materials did not involve any humans or animals.All methods were performed in accordance with the relevant guidelines and regulations.

Preparation and composition analysis of KSW
The KSW was provided by Qingdao Mingyue Company (Qingdao, Shandong).The KSW was obtained after 20 h of kelp soaking (kelp to water ratio 1:20) used in the production of alginate, algal functional sugar alcohols, algal functional food ingredients, etc.The components of the original solution of KSW were analyzed by Qingdao Feiyoute Testing Company (Qingdao, Shandong), and the findings are shown in Table 1.

Microbial inoculum preparation
Bacillus methylotrophicus M4-1 was isolated from the inter-rhizosphere soil of wheat in the saline zone of the Yellow River Delta, Shandong Province, China (118°49ʹ15″E, 37°24′31″N).It is a methylotrophic Bacillus strain, a gram-positive, facultatively aerobic bacterium.The detailed physiological and biochemical characteristics of this strain have been described by Ji et al. 78 .The NCBI accession number of M4-1 is MN938176.1.Strain M4-1 was used to inoculate Luria-Bertani LB liquid medium and cultured in a shaking incubator at 30 °C with 200 rpm for 12 h to obtain the seed solution.The seed solution was inoculated into a laboratoryoptimized fermentation medium (per liter of water: glucose, 20.0 g; peptone, 36.0 g; MgSO 4 , 3.2 g; K 2 HPO 4 , 5.6 g; KH 2 PO 4 , 2.8 g), at 2% (v/v) inoculum size, and incubated at 30 °C with 200 rpm shaking until the spore yield rate exceeded 90%.The spore yield rate was evaluated under an optical microscope (NIKON, Tokyo, JPN) with crystal violet staining 82 .The microbial pellet cells in the fermentation broth was collected and centrifuged at 4000 rpm for 30 min.The microbial pellet was resuspended in sterile deionized water for cell density of 1 × 10 9 cfu/mL 83 .

Pot experiment
Wheat cv jimai 21 seeds (provided by the College of Agriculture, Shandong Agricultural University) were washed with water, soaked in 75% ethanol for 10 min, then soaked in 30% sodium hypochlorite for 30-60 s, and finally washed 5-6 times with sterile water and dried.Uniform wheat seeds were selected and sown into pots (17 cm inner diameter), 12 seeds per pot.The soil used for the pot experiment were from an experimental field of Shandong Agricultural University (117°16ʹE, 36°17ʹN).After the wheat seedlings were approximately 10 cm long, the plants were watered with five different dilutions of KSW (20 mL) or the mixture of kelp-soaked wastewater at different dilutions and M4-1 bacterial suspension (1 × 10 9 cfu/mL, 10 mL), while the control group (CK) was only irrigated using 30 mL water.After that all groups were irrigated every 7 days with 30 mL water.Randomized complete block design was used in this study.
Kelp was soaked for 20 h according to the kelp to water ratio of 1:20 to obtain KSW stock solution.Measure 5 portions of KSW stock solution 0.1 L, add water 0.9 L, 1.9 L, 3.9 L, 7.9 L, 11.9 L respectively, and mix thoroughly to obtain the KSW with the dilution multiple of 10, 20, 40, 80 and 120 in order.The pH values of KSW with different dilutions (10, 20, 40, 80, 120) were 6.19, 6.46, 6.74, 6.97, 7.04, respectively.Six replicates were set up for each group.Potted were grouped as in Table 2.

Collection of wheat rhizosphere soil
After 35 days of treatment, the wheat seedlings were uprooted, and the roots were gently shaken to remove large soil particles.The soil tightly attached to the root surface was the rhizosphere soil 84 .The rhizosphere soil from

Extraction and PCR amplification of total soil microbial DNA
Total soil microbial DNA was extracted using FastDNA SPIN Kit for Soil.Purity of soil DNA was confirmed using 1% agarose gel electrophoresis.Quality-controlled soil DNA samples were sequenced by high-throughput sequencing.Three replicates were prepared for each treatment.Universal primers specific to the bacterial 16S rDNA V3-V4 region (338F, 5′-barcode-ACT CCT ACG GGA GGC AGC A-3′ and 806R, 5′-GGA CTA CHVGGG TWT CTAAT-3′) and to the fungal rDNA-ITS sequence (5′-barcode-CTT GGT CAT TTA GAG GAA GTAA-3′ and 2043R, 5′-GCT GCG TTC TTC ATC GAT GC-3′) were used for PCR amplification.PCR was set up as follows: 4 μL of 5 × FastPfu buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of positive primer (5 μM), 0.8 μL of negative primer (5 μM), 0.4 μL of fast-PFU polymerase, 10 ng template DNA 1, and ddH 2 O for a final volume of 20 μL.The reaction parameters for the amplification of the bacterial V3-V4 region were as follows: pre-denaturation at 95 °C for 2 min; 40 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 30 s; and final extension at 72 °C for 5 min.For the amplification of the fungal rDNA-ITS sequence, PCR was performed as follows: pre-denaturation at 95 °C for 3 min; 40 cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and extension at 72 °C for 45 s; and final extension at 72 °C for 10 min.Sample quality was examined by 2% agarose gel electrophoresis.The DNA bands were extracted and PCR products were recovered using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA).Finally, the PCR products were quantified using the QuantiFluor™-ST Blue Fluorescence Quantification System (Promega).Equimolar amounts of the purified amplicons were pooled and subjected to paired-end sequencing using an Illumina MiSeq instrument according to standard protocols.

Processing of sequencing data
Double-ended sequence data were obtained by MiSeq sequencing.First, pairs of reads were combined based on overlaps between the reads.QIIME (version 1.9.1) (http:// qiime.sourc eforge.net/) was used for quality read filtering.After the sample data were collated, OTU clustering at 97% similarity level and species taxonomic analyses were performed using UPARSE (version 7.1).Following OTU clustering, sample diversity indices were calculated using Mothur (version 1.30.2) software to compare the relative microbial diversity in samples.The 16S RNA read data were compared with information in the Silva (SSU123) 16S rRNA database, and RDP classifier was used to analyze 16S RNA reads in bacterial groups at different classification levels.The RDP classifier was also used to classify the ITS rDNA gene reads into fungal groups at different classification levels by comparing with UNITE 7.0/ITS database, at a confidence threshold of 0.7.UPGMA clustering analysis and PCoA were used to analyze the similarity between samples.Perl scripts were used to generate Venn diagrams to compare species composition of samples.

Determination of wheat growth, physical and chemical properties of the soil
The height and fresh weight of wheat seedlings in the treatment and control groups were determined.Thereafter, all wheat seedlings plants were baked in an oven at 80 °C until a constant weight was achieved as dry weight.
The available phosphorus (AP) was assayed by extraction with sodium bicarbonate 85 , Available potassium (AK) was assayed by the flame photometry method 86 , organic matter (OM) was assayed by the chromic acid titration method 87 , and alkali-hydrolyzed nitrogen was assayed by the alkali diffusion method.Soil pH was measured using a pH meter (METTLER TOLEDO, Shanghai, CHN).Activities of sucrase was determined by a colorimetric method using 3,5-dinitrosalicylic acid 88 .Urease was determined by sodium phenol-sodium Table 2. Group design of pot experiment and additives in each group.KSW kelp-soaking wastewater, -N/A.www.nature.com/scientificreports/hypochlorite colorimetric method 88,89 .Phosphatase was determined by benzene disodium phosphate colorimetric method 90 .Catalase was determined by potassium permanganate titration method 88,91 .

Statistical analysis
Data were expressed as the mean ± standard deviation (SD).Statistical analysis of plant and soil parameters was performed using ANOVA.In the process of one-way ANOVA, if the data do not meet the normal distribution, rank sum test is used.If variance is not uniform, Welch test is used.SPSS26.0 software (SPSS 26.0, SPSS, Chicago, IL, USA) was used for data statistics, differences in mean values were considered significant when p < 0.05. Vol

Figure 1 .
Figure 1.Effect of KSW, the mixture of KSW and M4-1 suspension on wheat seedlings.(a) Fresh weight, (b) Plant height, (c) Dry weight.Values are means ± SD (n = 6).Different letters indicate significant differences (p < 0.05) and the same letters indicate no significant differences (p > 0.05).

Figure 4 .
Figure 4. Venn diagram constructed at different OTUs levels of (a) bacteria and (b) fungus.

Figure 5 .
Figure 5. Composition and relative abundance of bacteria (a) and fungus (b) in different samples at the genus level.

Figure 6 .
Figure 6.Differences in community structure between groups.Relative abundance (%) of bacterial (a-c) and fungus (d-f) community composition at the phylum level among different treatments: (a,d) CK, (b,e) SE, (c,f) MS.(g) The significant differences in bacterial and fungal groups at the phylum level among the treatments (Student's t-test bar plot on phylum level).The rightmost is p value, *0.01 < p ≤ 0.05.

test items Results Detection limit Loq position Test method/basis
two pots of wheat seedlings combined, for three repeat samples.The rhizosphere soil was passed through a 2-mm sieve.A portion of the soil sample was air-dried for the determination of physical and chemical properties, and another portion was stored at − 80 °C for the extraction of soil DNA.Three replicates were prepared for each treatment.Wheat seedlings were collected for growth and biomass surveys, and each pot was used as a replicate with 6 replicates in each group.